| 货号 | AF3424-SP |
| 别名 | 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109 | 全称 | v-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human B-Raf in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 673 (Human); 114486 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human B-Raf Met1-His766 Accession # P15056 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: Raf-1 (C‑Raf); A-Raf; and B-Raf, found at high levels in cerebrum and testes. Mice with a targeted disruption of the B-Raf gene die of vascular defects during mid-gestation. B-Raf mutations have been found in two-thirds of malignant melanomas, with the single substitution V599E in the kinase domain the most frequent occurrence. B-Raf activation in melanomas results in BIM phosphorylation and inhibition of apoptosis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human B-Raf by Western Blot. Western blot shows lysates of T47D human breast cancer cell line, MDA‑MB‑453 human breast cancer cell line, Daudi human Burkitts lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B-Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for B-Raf at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human B‑Raf by Simple WesternTM. Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for B‑Raf at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human B‑Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. | |
Detection of Human B‑Raf by Simple WesternTM. Simple Western lane view shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for B‑Raf at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human B‑Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
