| 货号 | AF3416-SP |
| 别名 | DNA repair protein complementing XP-A cells; excision repair-controlling; Xeroderma pigmentosum group A-complementing protein; xeroderma pigmentosum, complementation group A; XP1; XPACXP1 | 全称 | Xeroderma Pigmentosum, Complementation Group A |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human XPA in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7507 (Human); 22590 (Mouse); 298074 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human XPA Met1-Met273 Accession # P23025 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TheXerodermaPigmentosumA (XPA) protein is a component of a large complex that binds to damaged DNA to facilitate nucleotideexcisionrepair (NER). XPA binds in a complex with ReplicationProteinA (RPA), XPG, and TFIIH (a subcomplex of the RNA polymerase II transcription initiation complex). XPA is thought to stabilize the binding of RPA to DNA, and thus aid in specific recognition of the damaged base. Because of their NER defect, XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human XPA by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPA Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3416) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPA at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5. |
