Human/Mouse/Rat Activin A Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat Activin A Affinity Purified Polyclonal Ab (25 UG)

货号： AF338-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF338-SP
别名	INHBA
反应种属	Human/Mouse/Rat
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse and rat Activin A in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Activin A-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. Schwall, R. H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND₅₀) is typically 2-6 µg/mL in the presence of 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3624 (Human); 16323 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps. Authors: Yang Y, Zhang N, Crombruggen K, Lan F, Hu G, Hong S, Bachert C PLoS ONE, 2015;10(6):e0128564. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice. Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V Am J Pathol, 2014;184(4):1152-66. Species: Mouse Sample Type: Muscle lysate Application: ELISA Development Fgf-2 overexpression increases excitability and seizure susceptibility but decreases seizure-induced cell loss. Authors: Zucchini S, Buzzi A, Barbieri M, Rodi D, Paradiso B, Binaschi A, Coffin JD, Marzola A, Cifelli P, Belluzzi O, Simonato M J. Neurosci., 2008;28(49):13112-24. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair. Authors: Barksby HE, Hui W, Wappler I, Peters HH, Milner JM, Richards CD, Cawston TE, Rowan AD Arthritis Rheum., 2006;54(2):540-50. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Activin A is a potent activator of renal interstitial fibroblasts. Authors: Yamashita S, Maeshima A, Kojima I, Nojima Y J. Am. Soc. Nephrol., 2004;15(1):91-101. Species: Canine Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Activin A
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786. Maeshima, A. et al. (2008) Endocr. J. 55:1. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534. Gray, A.M. and A.J. Mason (1990) Science 247:1328. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73. Onichtchouk, D. et al. (1999) Nature 401:480. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65. Schneyer, A. et al. (2003) Endocrinology 144:1671.

参考图片

Hemoglobin Expression Induced by Activin A and Neutralization by Human/Mouse/Rat Activin A Antibody. Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338). The ND₅₀ is typically 2‑6 µg/mL.

Activin A in Human Breast Cancer Tissue. Activin A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human/Mouse/Rat Activin A beta A Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF338) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.