| 货号 | AF3384-SP |
| 别名 | EC 2.7.10.1; EPH receptor B6; EphB6; ephrin type-B receptor 6; Hep; HEPTyrosine-protein kinase-defective receptor EPH-6; Mep; MGC129910; MGC129911 | 全称 | Eph Receptor B6 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human EphB6 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) EphB6 is observed and less than 1% cross-reactivity with recombinant human (rh) EphB4 and rhEphA2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2051 (Human); 13848 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human EphB6 Leu17 - Ser579 Accession # O15197 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | EphB6, also known as Hep and Mep, is a 110 kDa member of the Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by ligand preference and have a common structural organization (1‑4). The human EphB6 cDNA encodes a 1006 amino acid (aa) precursor that includes a 16 aa signal sequence, a 563 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 406 aa cytoplasmic domain. The ECD contains serine- and cysteine-rich regions and two fibronectin type-III domains. The cytoplasmic domain contains one non-catalytic protein kinase-like, one proline-rich, one SAM, and one PDZ-binding domain (5, 6). Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6. It shares 38‑45% aa sequence identity with human EphB1, 2, 3, 4, and 6. Human EphB5 has not been characterized. Two secreted splice variants have been described in mouse but not in human (6). EphB6 is primarily expressed in brain, pancreas, thymus, and peripheral T cells (5, 7, 8). EphB6 forms stable heterodimers with EphB1 and participates in signal transduction by association with other enzymatically active molecules (9‑11). Ephrin‑B2 is the dominant ligand for EphB6, although Ephrin‑B1 and Ephrin‑B3 can also trigger responses (12‑14). High concentrations of Ephrin‑B2 inhibit cell adhesion and migration as well as tyrosine phosphorylation of EphB6. Conversely, low concentrations of Ephrin‑B2 promote adhesion and migration and do not lead to EphB6 phosphorylation (15). The level of EphB6 expression is inversely correlated with tumor aggressiveness in a variety of malignancies (1). EphB6 also functions as a T cell co-stimulatory molecule (8, 11, 13). EphB6 clusters with the T cell receptor and participates in the subsequent attenuation of the T cell response (8, 10, 11, 13). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of EphB6 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Sheep Anti-Human EphB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3384, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). View our protocol for Staining Membrane-associated Proteins. |
