Human/Mouse/Rat SOX1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse/Rat SOX1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3369-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3369-SP
别名	SRY (sex determining region Y)-box 1; SRY-related HMG-box gene 1; transcription factor SOX-1
全称	SRY-related HMG-box 1
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human SOX1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) SOX2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6656 (Human); 20664 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A Stem Cells Transl Med, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: IHC Fresh Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells. Authors: Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S PLoS ONE, 2015;10(8):e0136350. Species: Human Sample Type: Whole Cells Application: IHC Not Specified Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Authors: Paull D, Sevilla A, Zhou H, Hahn A, Kim H, Napolitano C, Tsankov A, Shang L, Krumholz K, Jagadeesan P, Woodard C, Sun B, Vilboux T, Zimmer M, Forero E, Moroziewicz D, Martinez H, Malicdan M, Weiss K, Vensand L, Dusenberry C, Polus H, Sy K, Kahler D, Gahl W, Solomon S, Chang S, Meissner A, Eggan K, Noggle S Nat Methods, 2015;12(9):885-92. Species: Mouse Sample Type: Whole Cells Application: IHC Not Specified iPSC-Derived neural stem cells act via kinase inhibition to exert neuroprotective effects in spinal muscular atrophy with respiratory distress type 1. Authors: Simone C, Nizzardo M, Rizzo F, Ruggieri M, Riboldi G, Salani S, Bucchia M, Bresolin N, Comi G, Corti S Stem Cell Reports, 2014;3(2):297-311. Species: Human Sample Type: Whole Cells Application: IHC No Specified Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates. Authors: Villa-Diaz L, Kim J, Lahann J, Krebsbach P Stem Cells Transl Med, 2014;3(12):1410-7. Species: Human Sample Type: Whole Cells Application: ICC Sonic hedgehog signaling controls thalamic progenitor identity and nuclei specification in mice. Authors: Vue TY, Bluske K, Alishahi A, Yang LL, Koyano-Nakagawa N, Novitch B, Nakagawa Y J. Neurosci., 2009;29(14):4484-97. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human SOX1 Asn242-Gly379 (Leu276Ile) Accession # NP_005977
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SOX1 is a 39 kDa transcription factor that belongs to the SOXB1 subgroup. Within the developing CNS, SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Early Ectodermal Lineage Markers High Mobility Group (HMG) Transcription Factors ICC / IHC Images: SOX1 Neural Progenitor Cell Markers Neural Stem Cell Markers Neural Stem Cell Transcription Factors Pluripotent Stem Cell Transcription Factors

参考图片

SOX1 in differentiated NTera‑2 Human Cell Line. SOX1 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 in ectoderm differentuiated BG01V Human Embryonic Stem Cells. SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

SOX1 in Rat Cortical Stem Cells. SOX1 was detected in immersion fixed rat cortical stem cells (Catalog # NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX1 in Mouse Cortical Stem Cells. SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.