| 货号 | AF3367-SP |
| 别名 | protein Wnt-8b; wingless-type MMTV integration site family, member 8B | 全称 | Wingless-type MMTV Integration Site Family, Member 8b |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse Wnt-8b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Wnt-8a is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7479 (Human); 22423 (Mouse); 293990 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Wnt-8b Trp22-Glu52, Leu195-Arg269 Accession # Q9WUD6 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse Wnt-8b is a presumably 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 amino acids (aa) in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Wnt‑8b by Western Blot. Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Wnt‑8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3367) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑8b at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
