| 货号 | AF3364-SP |
| 别名 | paired box 2; paired box gene 2; paired box homeotic gene 2; paired box protein Pax-2 | 全称 | Paired Box Gene 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Pax2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax3, rhPax4, rhPax5, rhPax6, and rhPax7 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5076 (Human); 18504 (Mouse); 293992 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Histone deacetylase expression patterns in developing murine optic nerve. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Pax2 Asp229-Pro363 Accession # Q02962 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Pax2 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pax2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Pax2 in BG01V Human Embyonic Stem Cells. Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
