| 货号 | AF3360-SP |
| 别名 | BHLHA17; bHLHa17tal-1; Class A basic helix-loop-helix protein 17; SCLT-cell acute lymphocytic leukemia protein 1; Stem Cell Protein; Tal1; TAL-1; T-cell acute lymphocytic leukemia 1; T-cell leukemia/lymphoma protein 5; TCL5; TCL5tal-1 product | 全称 | Stem Cell Leukemia/T-cell Acute Lymphocytic Leukemia 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human SCL in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6886 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Inhibition of all-trans retinoic acid-induced granulocytic differentiation of WEHI-3B D+ cells by forced expression of SCL (TAL1) and GATA-1. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SCL Arg199-Gly266 Accession # P17542 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SCL, also known as TAL-1 (T-cell Acute Lymphocytic Leukemia-1 protein),is a 40 kDa basic helix-loop-helix transcription factor. It regulateshematopoiesis, angiogenesis and erythrocyte maturation. Activation ofSCL by chromosomal translocation may be a cause of some T-cell acutelymphoblastic leukemia. Two alternative splice variants lacking thefirst 25 or 175 amino acid residues also exist. Human and mouse SCLshare 93% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human SCL/Tal1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
