| 货号 | AF333-SP |
| 别名 | chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human CXCL6/GCP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse (rm) MIP-2, rmKC, rmCXCL6, recombinant human (rh) ENA-78, recombinant rat (rr) CINC-2 alpha, rmCRG-2, rhNAP-2 is observed and less than 5% cross-reactivity with rrCINC-2 beta and rhMCP-3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6372 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CXCL6/GCP-2 Val40-Asn114 Accession # P80162 |
| 内毒素水平 | <0.20 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GCP-2 (granulocyte chemotactic protein-2) also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level. Human GCP-2 cDNA encodes a propeptide of 114 amino acid residues with a predicted 37 aa residue signal peptide and 77 aa residue mature protein. Several forms of natural GCP-2 have been isolated from MG-63 conditioned media, indicating that GCP-2 undergoes limited processing at both the N- and C-termini. Human GCP-2 is a primary response gene whose induction by cytokines is attenuated by dexamethasone. Human GCP-2 and mouse GCP-2/LIX have been shown to chemoattract and activate neutrophils, but not eosinophils and monocytes. It is likely that GCP-2 activities are mediated via the human or mouse CXCR2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CXCL6/GCP‑2 and Neutralization by Human CXCL6/GCP‑2 Antibody. Recombinant Human CXCL6/ GCP‑2 (Catalog # 333-GC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL6/ GCP‑2 (80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL6/GCP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF333). The ND50 is typically 0.8‑4.0 µg/mL. |
