| 货号 | AF3334-SP |
| 别名 | B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4 | 全称 | B Lymphoma Mo-MLV Insertion Region 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human BMI-1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 648 (Human); 12151 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BMI-1 Asp96-Gly326 Accession # P35226 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BMI-1 (B cell-specific Moloney-MLV Integration site 1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of large multimeric complex termed PRC1 that inhibits target gene transcription loss of BMI-1 function and precludes stem cells from self-replicating. The amino acid sequence of human BMI-1 is 99%, 97%, 99%, and 99% identical to that of bovine, mouse, feline, and canine BMI-1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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BMI‑1 in K562 Human Cell Line. BMI‑1 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 µg/mL Goat Anti-Human BMI‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3334) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
