Human Aurora A Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Aurora A Affinity Purified Polyclonal Ab (25 UG)

货号： AF3295-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3295-SP
别名	AIK; AIKAurA; ARK1; ARK-1; ARK1EC 2.7.11.1; AURA; AURKA; aurora kinase AhARK1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora-related kinase 1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; BTAKAURORA2; EC 2.7.11; IPL1-related kinase; serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK15serine/threonine kinase 15; STK6; STK6MGC34538; STK7
反应种属	Human
应用	Western Blot(0.25 µg/mL) Simple Western(12.5 µg/mL)
目标/特异性	Detects human Aurora A in Western blots.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 12.5 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6790 (Human); 20878 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Kinome-wide RNAi studies in human multiple myeloma identify vulnerable kinase targets, including a lymphoid-restricted kinase, GRK6. Authors: Tiedemann RE, Zhu YX, Schmidt J, Yin H, Shi CX, Que Q, Basu G, Azorsa D, Perkins LM, Braggio E, Fonseca R, Bergsagel PL, Mousses S, Stewart AK Blood, 2010;115(8):1594-604. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Aurora A Met1-Glu131 Accession # O14965
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Aurora A is a serine/threonine kinase that regulates chromosome segregation and cytokinesis during mitosis. Its kinase activity is thought to regulate the G₂ to M transition of the cell cycle by phosphorylating BRCA1. Aberrant expression and activity of Aurora A in tumors, including breast, ovarian, gastric and colorectal cancer, suggests that it plays a role in tumor development and progression.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Cell Cycle Checkpoint Intracellular Kinases in the Akt Pathway Microtubules Stem Cell Epigenetics

参考图片

Detection of Human Aurora A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora A at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Detection of Human Aurora A by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Aurora A at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.