Human/Mouse/Rat Axin-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat Axin-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3287-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3287-SP
别名	axin 1; axin-1; AXINaxis inhibitor 1; Axis inhibition protein 1; fused, mouse, homolog of; hAxin; MGC52315
全称	Axis Inhibitor 1
反应种属	Human/Mouse/Rat
应用	Western Blot(0.5 µg/mL)
目标/特异性	Detects human, mouse and rat Axin-1 in Western blots.
使用方法	Western Blot: 0.5 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8312 (Human); 12005 (Mouse); 79257 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin. Authors: Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X Mol Cell Biol, 2015;35(8):1390-400. Species: Human Sample Type: Cell Lysates Application: IP Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning. EMBO J., 2012;31(16):3384-97. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of tankyrases induces Axin stabilization and blocks Wnt signalling in breast cancer cells. Authors: Bao R, Christova T, Song S, Angers S, Yan X, Attisano L PLoS ONE, 2012;7(11):e48670. Species: Human Sample Type: Cell Lysates Application: WB Canonical Wnt signaling negatively regulates platelet function. Authors: Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB Proc. Natl. Acad. Sci. U.S.A., 2009;106(47):19836-41. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Axin-1 Tyr210-Glu410 Accession # O15169
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.
运输条件	Blue Ice
存放说明	4℃
参考文献	Wnt Family Wnt Intracellular Signaling

参考图片

Detection of Human/Mouse/Rat Axin‑1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.