| 货号 | AF3284-SP |
| 别名 | E3 ubiquitin-protein ligase TRAF6; EC 6.3.2.-; Interleukin-1 signal transducer; RING finger protein 85; RNF85MGC:3310; TNF receptor-associated factor 6 | 全称 | TNF Receptor-Associated Factor 6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human TRAF-6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -3, -4, and -5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7189 (Human); 22034 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TRAF-6 Met1-Val522 Accession # Q9Y4K3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-6 is a 522 amino acid, 60 kDa protein. Overexpression of TRAF-6 mediates the activation of NF-kappa-B and JNK signaling pathways. TRAF-3 and TRAF-6 have been identified as adaptor molecules involved in TLR/IL-1R signaling events. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TRAF‑6 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, -TRAF5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-6 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
