Human CCL13/MCP-4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CCL13/MCP-4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF327-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF327-SP
别名	C-C motif chemokine 13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human CCL13/MCP-4 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) MCP-1, rhMCP-2, rhMCP-3, recombinant mouse (rm) MCP-5, rhEotaxin, rhHCC-1, rhMIP‑1 beta, rmMARC, rh6Ckine, and rmJE is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL13/MCP‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 15-40 µg/mL in the presence of 2 µg/mL Recombinant Human CCL13/MCP‑4.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6357 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CCL13 is a promising diagnostic marker for systemic sclerosis. Authors: Yanaba K, Yoshizaki A, Muroi E, Hara T, Ogawa F, Shimizu K, Hasegawa M, Fujimoto M, Takehara K, Sato S Br. J. Dermatol., 2010;162(2):332-6. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces. Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C Eur J Immunol, 2002;32(1):231-42. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas. Authors: Maggio EM, Van Den Berg A, Visser L, Diepstra A, Kluiver J, Emmens R, Poppema S Int. J. Cancer, 2002;99(5):665-72. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CCL13/MCP-4 (R&D Systems, Catalog # 327-P4) Gln24-Thr98 Accession # Q99616
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4⁺ and CD8⁺ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma MCP-4 and Mouse MCP-5: New C-C Chemokines

参考图片

Chemotaxis Induced by CCL13/MCP‑4 and Neutralization by Human CCL13/MCP‑4 Antibody.

Recombinant Human CCL13/MCP‑4 (Catalog # 327‑P4) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP‑4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL13/MCP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF327). The ND₅₀ is typically 15‑40 µg/mL.