| 货号 | AF3279-SP |
| 别名 | CART1; CART1RING finger protein 83; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; MLN62; MLN62cysteine-rich domain associated with ring and TRAF domain; RNF83malignant 62; TNF receptor-associated factor 4; tumor necrosis receptor-associated factor 4A | 全称 | TNF Receptor-Associated Factor 4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human TRAF-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -3, -5, or -6 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9618 (Human); 22032 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TRAF-4 Met1-Ser470 Accession # Q9BUZ4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-4, also known as CART1 and MLN62, is a 470 amino acid, 53 kDa protein. TRAF4 is unique member of the TRAF family that localizes to the nucleus and does not regulate signaling through cell surface receptors. TRAF-4 is highly expressed in human breast carcinomas and during embryogenisis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TRAF‑4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitts lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human TRAF‑1, TRAF‑2, TRAF‑3, TRAF‑4, TRAF‑5, and TRAF‑6 (2 ng/lane) were included. A specific band for TRAF-4 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
