| 货号 | AF3278-SP |
| 别名 | CAP1; CAP-1CD40 receptor associated factor 1; CD40 associated protein 1; CD40 binding protein; CD40 receptor-associated factor 1; CD40-binding protein; CD40bp; CRAF1; CRAF1CAP1; LAP1; LAP1CD40BP; LMP1-associated protein 1; TNF receptor-associated factor 3 | 全称 | TNF Receptor-Associated Factor 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human TRAF-3 Isoform 2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -4, -5, or -6 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7187 (Human); 22031 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TRAF-3 Met1-Pro543 Accession # EAW81797 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C‑terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N‑terminal RING and zinc finger domains that are involved in signaling downstream events. Two isoforms of TRAF-3 have been identified, a 368 amino acid, 64 kDa protein (isoform 1, also known as CD40BP and CRAF1) and a 543 amino acid, 61 kDa protein (isoform 2, also known as CAP1 and LAP1). TRAF‑3 has been shown bind specifically to the PEQET sequence motif on the cytoplasmic domain of CD40. TRAF3 and TRAF6 have also been identified as being involved in TLR signaling events. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TRAF‑3 Isoform 2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF‑3 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human TRAF‑1, TRAF-2, TRAF-3, TRAF-4, TRAF‑5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
