| 货号 | AF3265-SP |
| 别名 | CAK1 antigen; CAK1; megakaryocyte potentiating factor; mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Mesothelin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10232 (Human); 56047 (Mouse); 60333 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Mesothelin isoform 2 Glu296-Gly580 Accession # ABW03459 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mesothelin is a 40 kDa, 292 amino acid (aa) GPI-linked glycoproteinfound on normal mesothelial cells. It is also highly expressed inpancreatic, gastric and ovarian carcinomas. Mesothelin is synthesizedas a preproprotein with a carboxy-terminal hydrophobic proregion thatis removed when a GPI-anchor is added. The N-terminal prosegment iscleaved by furin to release a 33 kDa soluble protein that is also knownas megakaryocyte potentiating factor (MPF). The membrane bound portionof Mesothelin can also be shed and is detectable in sera. Within theregion used as the immunogen, human Mesothelin shares approximately 58%amino acid sequence identity with the mouse or rat protein. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Mesothelin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Mesothelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mesothelin at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and Immunoblot Buffer Group 8. |
