Human VEGF R1/Flt-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human VEGF R1/Flt-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF321-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF321-SP
别名	EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGFR1; VEGFR-1
全称	Vascular Endothelial Growth Factor Receptor 1
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse VEGF R1 and less than 10% cross-reactivity with recombinant human (rh) VEGF R2 and rhVEGF R3.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human PlGF (Catalog # 264-PG) to immobilized Recombinant Human VEGF R1/Flt-1 Fc Chimera (Catalog # 321-FL) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2321 (Human); 14254 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration. Authors: Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S PLoS ONE, 2015;10(9):e0138587. Species: Human Sample Type: Whole Cells Application: Neut Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice. Authors: Szalai G, Romero R, Chaiworapongsa T, Xu Y, Wang B, Ahn H, Xu Z, Chiang P, Sundell B, Wang R, Jiang Y, Plazyo O, Olive M, Tarca A, Dong Z, Qureshi F, Papp Z, Hassan S, Hernandez-Andrade E, Than N PLoS ONE, 2015;10(4):e0119547. Species: Human Sample Type: Cell Lysates Application: WB Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis. Authors: Hou H, Cheng S, Chung K, Kuo M, Yeh C, Chang B, Lu H, Wang H, Yu C Autophagy, 2014;10(9):1509-21. Species: Human Sample Type: Whole Cells Application: Neut Melanoma spheroid formation involves laminin-associated vasculogenic mimicry. Authors: Larson A, Lee C, Lezcano C, Zhan Q, Huang J, Fischer A, Murphy G Am J Pathol, 2014;184(1):71-8. Species: Human Sample Type: whole cells Application: IHC Merkel cell carcinoma expresses vasculogenic mimicry: demonstration in patients and experimental manipulation in xenografts. Authors: Lezcano C, Kleffel S, Lee N, Larson A, Zhan Q, DoRosario A, Wang L, Schatton T, Murphy G Lab Invest, 2014;94(10):1092-102. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Attenuation of VEGFR-2 expression by sFlt-1 and low oxygen in human placenta. Authors: Nevo, Ori, Lee, Dennis K, Caniggia, Isabella PLoS ONE, 2013;8(11):e81176. Species: Human Sample Type: tissue Application: Neut Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors. Authors: Vizio, Barbara, Biasi, Fiorella, Scirelli, Tiziana, Novarino, Anna, Prati, Adriana, Ciuffreda, Libero, Montrucchio, Giuseppe, Poli, Giuseppe, Bellone, Graziell J Transl Med, 2013;11(0):314. Species: Human Sample Type: Whole Cells Application: Blocking Differential expression of Vegfr-2 and its soluble form in preeclampsia. Authors: Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM PLoS ONE, 2012;7(3):e33475. Species: Human Sample Type: Plasma Application: WB gamma-Secretase and presenilin mediate cleavage and phosphorylation of vascular endothelial growth factor receptor-1. Authors: Cai J, Chen Z, Ruan Q, Han S, Liu L, Qi X, Boye SL, Hauswirth WW, Grant MB, Boulton ME J. Biol. Chem., 2012;286(49):42514-23. Species: Bovine Sample Type: Whole Cells Application: Proximity Ligation Assay Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues. Authors: Barsoum IB, Renaud SJ, Graham CH Am. J. Pathol., 2011;178(6):2888-96. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt. Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7. Species: Human Sample Type: Whole Cells Application: ICC Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis. Authors: Lee HS, Myers A, Kim J Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67. Species: Human Sample Type: Whole Cells Application: Neut Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Authors: Ball SG, Shuttleworth CA, Kielty CM J. Cell Biol., 2007;177(3):489-500. Species: Human Sample Type: Whole Cells Application: Neut Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Am. J. Pathol., 2006;169(4):1167-82. Species: Human Sample Type: Whole Cells Application: Neut Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals. Authors: Akintoye SO, Lam T, Shi S, Brahim J, Collins MT, Robey PG Bone, 2006;38(6):758-68. Species: Human Sample Type: Whole Cells Application: ICC Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro. Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L Chem. Biol., 2006;13(5):549-56. Species: Human Sample Type: Whole Cells Application: Flow Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7. Species: Human Sample Type: Whole Cells Application: ICC All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells. Authors: Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E Leuk. Res., 2004;28(3):243-8. Species: Human Sample Type: Whole Cells Application: ICC PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis. Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A Exp. Cell Res., 2004;299(1):68-78. Species: Human Sample Type: Whole Cells Application: Neut Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V Blood, 2003;102(2):638-45. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1 Ser27-His687 Accession # NP_001153392
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Cancer Biomarkers Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Progenitor and Endothelial Cell Markers HIF Transcription Factors ICC / IHC Images: VEGF R1/Flt-1 Mini-review: Vascular Endothelial Growth Factor (VEGF) Myeloid-derived Suppressor Cells (MDSC) Platelets Preeclampsia Proliferation Bioassay HUVECs Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway Technical Notes: The Quantikine ELISA in Establishing a Role for Endoglin in Preeclampsia VEGF Family

参考图片

VEGF R1/Flt‑1 in Human Breast Cancer Tissue. VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

VEGF R1/Flt‑1 in Human Ovarian Cancer Tissue. VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.