Human IKK epsilon Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IKK epsilon Affinity Purified Polyclonal Ab (25 UG)

货号： AF3199-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3199-SP
别名	EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase epsilon; IKBKE; IKK-E; IKKEMGC125297; IKK-epsilon; IKKI; IKK-I; IKK-iMGC125295; Inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon; KIAA0151IKK-related kinase epsilon; MGC125294
全称	IkB Kinase epsilon
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(10-25 µg/mL)
目标/特异性	Detects human IKK epsilon in Western blots.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 10-25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9641 (Human); 56489 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IKK epsilon Met1-Val716 Accession # Q14164
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	I kappa B kinase epsilon (IKK epsilon ) is also known as IKBKE and IKKi. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK epsilon is an IKK-related kinase with 30% structural homology to IKK alpha and beta, and is present in a subset of IKK complexes. IKK epsilon activation has been implicated in the initiation of anti-viral gene expression and in the pathogenesis of breast cancer.
运输条件	Blue Ice
存放说明	4℃
参考文献	C-type Lectin Receptors ICC / IHC Images: IKK epsilon IL-1 Family IL-17 Family Intracellular Kinases Intracellular Kinases in the Akt Pathway Natural Killer T (NKT) Cells NFkB Pathway NOD-like Receptors and the Inflammasome Notch Pathway Positive Regulators of the Jak/STAT Pathway RIG-I-like Receptors Toll-Like Receptors

参考图片

Detection of Human IKK epsilon by Western Blot. Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line, Raji human Burkitts lymphoma cell line, and Ramos human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IKK epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IKK epsilon at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IKK epsilon in Ramos Human Cell Line. IKK epsilon was detected in immersion fixed Ramos human Burkitts lymphoma cell line using Goat Anti-Human IKK epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.