| 货号 | AF3196-SP |
| 别名 | DKFZp686F03189; EC 2.7.11; EC 2.7.11.24; ERK-3; ERK3p97-MAPK; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; mitogen-activated protein kinase 6; p97MAPK; PRKM6; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6 | 全称 | Extracellular Signal-regulated Kinase 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human ERK3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5597 (Human); 50772 (Mouse); 58840 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ERK3 Met344-Asn721 Accession # Q16659 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Extracellular signal-regulated kinase 3 (ERK3) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 6 (MAPK6) and p97MAPK. Unlike ERK1 and ERK2, ERK3 lacks the phosphoacceptor sequence T-E-Y in its activation loop. This kinase also possesses a unique C-terminal extension that is missing in traditional MAPKs. Highly unstable, ERK3 is rapidly degraded following N-terminal ubiquitination. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human ERK3 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human ERK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK3 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
