| 货号 | AF3194-SP |
| 别名 | CIS-1cytokine-inducible SH2-containing protein; CIScytokine-inducible inhibitor of signaling type 1B; CISH; cytokine inducible SH2-containing protein; G18Protein G18; SOCS; Suppressor of cytokine signaling | 全称 | Cytokine Inducible SH2-containing Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human CIS-1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1154 (Human); 12700 (Mouse); 83681 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CIS-1 Leu11-Leu258 Accession # Q9NSE2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cytokine Inducible SH2-containing protein (CIS-1) is a 29 kDa protein found in a variety of cell types. Mono or polyubiquitination generally results in a 37 or 45 kDa molecule. CIS-1 binds to phosphorylated cytokine receptors IL-3 R beta and EPO-R and blocks downstream activation of STAT5 via receptor internalization and ubiquitin‑mediated proteosomal degradation. Human CIS-1 is a 258 aa peptide that contains one SH2 domain (aa 82‑163) and one SOCS box (aa 218‑258). There are two known alternatively spliced variants with a 7- or 13-aa substitution for the 7 N-terminal amino acid residues. Over the region used as immunogen, human CIS-1 is 91% identical to the corresponding mouse and canine protein sequences. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CIS-1 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CIS-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CIS-1 at approximately 35‑40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
