| 货号 | AF3167-SP |
| 别名 | C1q and tumor necrosis factor related protein 5; Complement C1q Tumor Necrosis Factor-Related Protein 5 Precursor Variant 3; CTRP5; CTRP5LORDDKFZp586B0621; LORD 6; Myonectin 3 | 全称 | C1q And Tumor Necrosis Factor Related Protein 5 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/mL) |
| 目标/特异性 | Detects human C1qTNF5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human C1qTNF4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 114902 (Human); 235312 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human C1qTNF5 Ser16-Ala243 Accession # AAH29485 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | C1qTNF5, also known as CTPR5, is a secreted protein that belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF-related protein family. All family members share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a C‑terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse C1qTNF5 share 94% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of C1qTNF5 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human C1qTNF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3167, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
