| 货号 | AF3166-SP |
| 别名 | B-FABP; B-FABPDKFZp547J2313; BLBP; BLBPMRG; Brain lipid-binding protein; Brain-type fatty acid-binding protein; FABPBbrain lipid binding protein; fatty acid binding protein 7, brain; Fatty acid-binding protein 7; fatty acid-binding protein, brain; Mammary-derived growth inhibitor related; mammary-derived growth inhibitor-related | 全称 | Fatty Acid-Binding Protein 7 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Simple Western(50 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FABP7/B-FABP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP6, rhFABP8, rhFABP9 and recombinant mouse FABP9 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Simple Western: 50 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2173 (Human); 12140 (Mouse); 80841 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FABP7/B-FABP Met1-Ala132 Accession # O15540 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FABP7, also known as (Brain) B-FABP, Brain lipid-binding protein (BLBP), and mammary-derived growth inhibitor-related (MRG), belongs to the family of intracellular lipid-binding proteins. It is involved in fatty acid uptake and intracellular transport and is important in brain development. Human and mouse FABP7 share 86% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FABP7 in Human Melanoma. FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human FABP7/B‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human cerebellum tissue and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP7/B‑FABP at approximately 18 kDa (as indicated) using 50 µg/mL of Goat Anti-Human FABP7/B‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
