Human IL-15 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-15 Affinity Purified Polyclonal Ab (25 UG)

货号： AF315-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF315-SP
别名	IL-15MGC9721; interleukin 15; interleukin-15
全称	Interleukin 15
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑15 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line [Avanzi, G. et al. (1988) Br. J. Haematol. 69:359]. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 10 ng/mL of Recombinant Human IL‑15.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells. Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G Gene Ther, 2015;22(1):76-86. Species: Human Sample Type: Cell Lysates Application: WB TLR-9 and IL-15 Synergy Promotes the In Vitro Clonal Expansion of Chronic Lymphocytic Leukemia B Cells. Authors: Mongini P, Gupta R, Boyle E, Nieto J, Lee H, Stein J, Bandovic J, Stankovic T, Barrientos J, Kolitz J, Allen S, Rai K, Chu C, Chiorazzi N J Immunol, 2015;195(3):901-23. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis. Authors: Broux B, Mizee M, Vanheusden M, van der Pol S, van Horssen J, Van Wijmeersch B, Somers V, de Vries H, Stinissen P, Hellings N J Immunol, 2015;194(5):2099-109. Species: Human Sample Type: Whole Tissue Application: IHC- Frozen Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment. Authors: Marcondes MC, Burdo TH, Sopper S, Huitron-Resendiz S, Lanigan C, Watry D, Flynn C, Zandonatti M, Fox HS J. Immunol., 2007;178(9):5812-9. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Tissue Application: IHC Paraffin-embedded Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism. Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D J. Immunol., 2005;174(4):2376-86. Species: Human Sample Type: Whole Cells Application: Neut Cloning and expression of feline interleukin 15. Authors: Dean GA, Barger A, Lavoy A Cytokine, 2005;29(2):77-83. Species: Feline Sample Type: Whole Cells Application: ICC NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen. Authors: Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF J. Immunol., 2004;172(1):130-7. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-15 Asn49-Ser162 Accession # P40933
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8⁺ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Grabstein, K. et al. (1994) Science 264:965. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444. Giri, J.G. et al. (1995) EMBO 14:3654. Giri, J. et al. (1994) EMBO J. 13:2822. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851. Dubois, S. et al. (2002) Immunity 17:537. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. Mortier, E. et al. (2004) J. Immunol. 173:1681. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

参考图片

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (10 ) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315). The ND₅₀ is typically 0.5-1.5 µg/mL.

IL‑15 in Human Tonsil. IL‑15 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.