| 货号 | AF3157-SP |
| 别名 | MGC138165; MGC141991; Protein Wnt-14; wingless-type MMTV integration site family, member 14; wingless-type MMTV integration site family, member 9A; Wnt-14; WNT14protein Wnt-9a | 全称 | Wingless-type MMTV Integration Site Family, Member 9A |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑9b is observed and less than 1% cross-reactivity with rmWnt-1 and rmWnt-4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7483 (Human); 216795 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Wnt‑9a Pro48-Glu92 & Arg231-Phe304 Accession # O14904.2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Wnt-9a (Wingless related integration site family member 9a, formerly Wnt-14) is a member of the Wnt family, which are ligands for Frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL and gastric, pancreatic, and breast cancer cells. Secretion of Wnt-9a is believed to play a role in avian atrioventricular cardiac cushion development. Human Wnt-9a is 98% amino acid (aa) identical with both mouse and rat Wnt-9a. The Wnts make up a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt family members vary in length between 350 and 400 aa, possess 22 to 24 conserved cysteines, are highly hydrophobic, and show 20 - 85% aa identity within the Wnt family. In general, there are three signaling pathways associated with Wnt-receptor interaction. The first is commonly called the canonical pathway and ultimately culminates in beta-Catenin accumulation and TCF/LEF-1-mediated gene transcription. The other two less well-defined non-canonical pathways include the Wnt/Ca2+ pathway and the planar cell polarity (PCP) pathway. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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