| 货号 | AF3156-SP |
| 别名 | AGT; alpha-1 antiproteinase, antitrypsin; Angiotensin II; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Angiotensinogen; ANHU; Aogen; FLJ92595; FLJ97926; PAT; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; SERPINA8angiotensin I |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(2-15 µg/mL) |
| 目标/特异性 | Detects human Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Serpin A8/Angiotensinogen is observed, and less than 5% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 2-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 183 (Human); 11606 (Mouse); 24179 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The inflammatory response in the MPTP model of Parkinson's disease is mediated by brain angiotensin: relevance to progression of the disease. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Serpin A8/Angiotensinogen Asp34-Ala485 Accession # ABM85122 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Serpin A8 (serineproteinaseinhibitor-cladeA #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II. Ang II induces vascoconstriction and aldosterone release. Mature human angiotensinogen is 452 aa in length (aa 34-485). It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41). Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Serpin A8/Angiotensinogen by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin A8/Angiotensinogen at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Serpin A8/Angiotensinogen in Human Brain. Serpin A8/Angiotensinogen was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Serpin A8/Angiotensinogen by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin A8/Angiotensinogen at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
