| 货号 | AF3150-SP |
| 别名 | Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; A-FABP; A-FABPAFABP; ALBP; aP2; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte | 全称 | Fatty Acid-Binding Protein 4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FABP4/A‑FABP in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse (rm) FABP4 is observed, approximately 30% cross‑reactivity with recombinant human (rh) FABP3 is observed, and less than 5% cross‑reactivity with rhFABP1, -2, -5, -6, -7, -8, -9, and rmFABP9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2167 (Human); 11770 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FABP4/A‑FABP Cys2-Ala132 Accession # P15090 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat FABP4/A‑FABP by Western Blot. Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FABP4/A‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
FABP4/A‑FABP in Human Bladder Cancer Tissue. FABP4/A‑FABP was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human and Mouse FABP4/A‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 19 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
