Human Frizzled-6 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Frizzled-6 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3149-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3149-SP
别名	frizzled (Drosophila) homolog 6; frizzled homolog 6 (Drosophila); frizzled-6; FZ6; fz-6; FZD6; Hfz6; seven transmembrane helix receptor
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Frizzled-6 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Frizzled-6 is observed, and less than 5% cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, recombinant human (rh) Frizzled-5, rmFrizzled-7, rmFrizzled-8, and rhFrizzled-10 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8323 (Human); 14368 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines. Authors: Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A BMC Cancer, 2013;13(0):557. Species: Human Sample Type: whole cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Frizzled-6 His19-Val153 Accession # O60353
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Frizzled-6 is a member of the Frizzled/Smoothened family of G-proteincoupled receptors that mediate the Wnt beta-Catenin signaling pathway.
运输条件	Blue Ice
存放说明	4℃
参考文献	Frizzled Receptors ICC / IHC Images: Frizzled-6 Mini-review: Wnt Receptors & Pathways Ryk: A Wnt Co-receptor at the Intersection of Frizzled & Dishevelled Technical Notes: Wnt Inhibitor Activity Wnt Family Wnt Receptors Wnt Signaling and sFRPs

参考图片

Frizzled‑6 in Human Kidney Cancer Tissue. Frizzled‑6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.