| 货号 | AF3135-SP |
| 别名 | f-spondin; KIAA0762VSGP/F-spondin; MGC10724; SPON1; spondin 1, (f-spondin) extracellular matrix protein; spondin 1, extracellular matrix protein; spondin-1; Vascular smooth muscle cell growth-promoting factor; VSGP |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human F‑Spondin/SPON1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize the the enhancemment of neurite outgrowth of dorsal root ganglion neurons from E13 chick embryos induced by F‑Spondin. 3 µg/mL is sufficient to block neurite outgrowth induced by 90 ng/3 μL drop/well of F-Spondin immobilized on nitrocellulose coated plate. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10418 (Human); 233744 (Mouse); 64456 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human F‑Spondin/SPON1 Phe29-Cys807 Accession # Q9HCB6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | F-Spondin (“Floor plate” and “thrombospondin” homology; also Spondin-1 and VSGP) is a member of the F-Spondin family of proteins that collectively belong to a subgroup of TSR (thrombospondin) type I class molecules (1). Class I molecules are either membrane-bound or ECM-associated. F-Spondin is a 110 kDa, secreted, heparin-binding extracellular matrix glycoprotein first characterized in rat for its high expression in embryonic floor plate (2‑4). Human F-Spondin is synthesized as an 807 amino acid (aa) precursor that contains a 28 aa signal sequence and a 779 aa mature region (3, 4). The mature region includes an N-terminal reelin-like domain (aa 1‑200), a centrally placed F-Spondin (FS) type segment (aa 201‑440), and six C-terminal class 2 thrombospondin type I repeats (1, 3, 5). Class 1 and 2 repeats differ in the placement of their cysteine residues. The fifth and sixth TSP repeats (aa 668‑806) apparently bind ECM, while TSP repeats 1‑4 (aa 442‑666), plus the spondin segment, are suggested to mediate either repulsive activity (on motor neurons), or outgrowth promoting activity (on sensory neurons) (1, 6). At least two isoforms of F-Spondin are known. Both are proteolytically-generated, one by plasmin, another by an unidentified protease. Plasmin cleaves the C-terminus at two points, generating a soluble, 95 kDa, 656 aa F‑Spondin that contains all but TSP repeats #5 and 6 (7). The unidentified protease appears to cleave F-Spondin between the FS segment and the first TSP repeat, generating 60 kDa and 50 kDa fragments, respectively (6). F-Spondin shows highly unusual glycosylation, exhibiting both C-mannosylation (mannose bound to Trp) and O-fucosylation (fucose bound to Ser/Thr) (4). The significance of these glycosidic modifications is unknown. Mature human F-Spondin is 98%, 97%, 98%, and 97% aa identical to mature canine, rat, bovine and mouse F-Spondin, respectively. Mammalian cells known to express F‑Spondin include floor plate epithelium, ventral motor neurons, Schwann cells, fibroblasts, hippocampal pyramidal cells, endothelial cells, vascular smooth muscle cells and some tumor cells (6, 8, 9). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human F‑Spondin/SPON1 by Western Blot. Western blot shows lysates of OVCAR‑3 human ovarian carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human F‑Spondin/SPON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3135) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for F‑Spondin/ SPON1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
