Human COMP Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human COMP Affinity Purified Polyclonal Ab (25 UG)

货号： AF3134-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3134-SP
别名	cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin-5; TSP5
全称	Cartilage Oligomeric Matrix Protein
反应种属	Human
应用	Western Blot(0.05 µg/mL)
目标/特异性	Detects human COMP in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Thrombospondin-1, -2, and -4 is observed.
使用方法	Western Blot: 0.05 µg/mL Neutralization: Measured by its ability to neutralize COMP/Thrombospondin‑5-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 10 µg/mL Recombinant Human COMP/Thrombospondin‑5.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1311 (Human); 12845 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human COMP/Thrombospondin-5 Gln21-Ala757 (Ala256Arg) Accession # P49747
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous coiled‑coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D₃ which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961. Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294. Newton, G. et al. (1994) Genomics, 24:435. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422. Efimov, V.P. et al. (1994) FEBS Lett. 341:54. Ozbek, S. et al. (2002) EMBO J. 21:5960. Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207. Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547. Hou, J. et al. (2000) Cell Calcium 27:309.

参考图片

Detection of Human COMP/Thrombospondin‑5 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human COMP/Thrombospondin‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for COMP/Thrombospondin‑5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Adhesion Mediated by COMP/Thrombospondin‑5 and Neutralization by Human COMP/Thrombospondin‑5 Antibody. Recombinant Human COMP/Thrombospondin‑5 (Catalog # 3134-CP), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human COMP/Thrombospondin‑5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human COMP/Thrombospondin‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134). The ND₅₀ is typically
1-4 µg/mL.