| 货号 | AF3114-SP |
| 别名 | CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; DECTIN2; dectin-2; Dendritic cell-associated C-type lectin 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human Dectin‑2/CLEC6A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Dectin-2 alpha is observed and less than 1% cross-reactivity with recombinant human Dectin-1/CLEC7A is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 93978 (Human); 56620 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Dectin‑2/CLEC6A Thr46-Leu209 Accession # Q6EIG7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4+ T cells (4‑6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is up‑regulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4+CD25+ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Dectin‑2/CLEC6A in Human Monocytes by Flow Cytometry. Human blood-derived monocytes were stained with Goat Anti-Human Dectin‑2/CLEC6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3114, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). |
