Human CXCL12/SDF-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human CXCL12/SDF-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-310-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-310-SP
别名	chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1
反应种属	Human
应用	Western Blot,Neutralization
目标/特异性	Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND₅₀) is typically 1.5-6.0 µg/mL in the presence of 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dual targeting of the chemokine receptors CXCR4 and ACKR3 with novel engineered chemokines. Authors: Hanes M, Salanga C, Chowdry A, Comerford I, McColl S, Kufareva I, Handel T J Biol Chem, 2015;290(37):22385-97. Species: Human Sample Type: Virus Application: ELISA Capture COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration. Authors: Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F BMC Cancer, 2014;14(0):407. Species: Human Sample Type: Cell Lysates Application: WB A paracrine network regulates the cross-talk between human lung stem cells and the stroma. Authors: Ruiz E, Oeztuerk-Winder F, Ventura J Nat Commun, 2014;5(0):3175. Species: Human Sample Type: Cell Lysates Application: WB Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node. Authors: Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M J Immunol, 2014;192(5):2133-42. Identification and characterization of circulating variants of CXCL12 from human plasma: effects on chemotaxis and mobilization of hematopoietic stem and progenitor cells. Authors: Richter R, Jochheim-Richter A, Ciuculescu F, Kollar K, Seifried E, Forssmann U, Verzijl D, Smit M, Blanchet X, von Hundelshausen P, Weber C, Forssmann W, Henschler R Stem Cells Dev, 2014;23(16):1959-74. Species: Human Sample Type: Plasma Application: Functional Assay CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis. Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S Eur. J. Immunol., 2008;38(11):3050-9. Species: Human Sample Type: Whole Cells Application: Neut Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice. Authors: Brzoska E, Grabowska I, Hoser G, Streminska W, Wasilewska D, Machaj EK, Pojda Z, Moraczewski J, Kawiak J Exp. Hematol., 2006;34(9):1262-70. Species: Human Sample Type: Whole Tissue Application: IHC Functional gene expression analysis of clonal plasma cells identifies a unique molecular profile for light chain amyloidosis. Authors: Abraham RS, Ballman KV, Dispenzieri A, Grill DE, Manske MK, Price-Troska TL, Paz NG, Gertz MA, Fonseca R Blood, 2005;105(2):794-803. Species: Human Sample Type: Whole Cells Application: ICC A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. Authors: Carman CV, Springer TA J. Cell Biol., 2004;167(2):377-88. Species: Human Sample Type: Whole Cells Application: ICC Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Authors: Casazza S, Zappia E, Pistorio A, Gambini C Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. Authors: Carman CV, Springer TA J. Cell Biol., 2004;167(2):377-88. Species: Human Sample Type: Whole Cells Application: ICC CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells. Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O J. Immunol., 2004;173(6):3668-75. Species: Mouse Sample Type: Whole Cells Application: Neut Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A J. Leukoc. Biol., 2003;73(5):584-90. Species: Human Sample Type: Whole Cells Application: Neut Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice. Authors: Cashman J, Clark-Lewis I, Eaves A, Eaves C Blood, 2002;99(3):792-9. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CXCL12/SDF‑1 alpha Lys22-Lys89 Accession # Q6ICW0
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion Chemokines and Receptors Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors HIF Transcription Factors Infectious Diseases Megakaryocytes Neuroinflammation Regulatory T Cells (Tregs)

参考图片

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCL12/SDF‑1 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human
CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND₅₀ is typically 1.5-6.0 µg/mL.