| 货号 | AF3108-SP |
| 别名 | NEFH neurofilament, heavy polypeptide; NEFH; neurofilament, heavy polypeptid; NFH | 全称 | Neurofilament Protein, Heavy Chain |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human NF-H in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) NF-M and rhNF-L is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4744 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NF-H Met1-Ala380 Accession # P12036 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neurofilament (NF)-H is a 200 kDa neurofilament protein and is one ofthree NF subunits. In the protein region used for immunization, humanNF-H shares approximately 92% sequence identity with the mouse or ratprotein. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
NF-H in Human Brain. NF-H was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Goat Anti-Human NF-H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
