| 货号 | AF3080-SP |
| 别名 | A33; cell surface A33 antigen; glycoprotein A33 (transmembrane); Glycoprotein A33; GPA33; MGC129986; MGC129987; transmembrane glycoprotein A33 | 全称 | Cell Surface A33 Antigen |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10223 (Human); 59290 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human A33 Ile22-Val235 Accession # Q99795 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2, and JAM-A, -B, and -C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD shares 80%, 74%, and 71% aa sequence identity with canine, bovine, and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of A33 in TH‑29 Human Cell Line by Flow Cytometry. TH‑29 human colon carcinoma cell line was stained with Human A33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3080, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). | |
A33 in Human Colon. A33 was detected in immersion fixed paraffin-embedded sections of human colon using 1.7 µg/mL Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
