| 货号 | AF3077-SP |
| 别名 | EFABP; E-FABP; E-FABPPAFABP; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog | 全称 | Fatty Acid-Binding Protein 5 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Simple Western(12.5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) FABP5 is observed and less than 5% cross-reactivity with recombinant human FABP1, -2, -3, -4, -6, -7, and rmFABP9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 12.5 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2171 (Human); 16592 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Curcumin restores sensitivity to retinoic acid in triple negative breast cancer cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FABP5/E‑FABP Ala2-Glu135 Accession # Q01469 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human FABP-5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FABP5/E‑FABP in HUVEC Human Umbilical Vein Endothelial Cells. FABP5/E‑FABP was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
Detection of Human FABP5/E‑FABP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at approximately 21 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
