Human SOX9 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human SOX9 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3075-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3075-SP
别名	campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9
全称	Transcription Factor SOX9
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) SOX10 is observed, and less than 15% rhSOX11, rhSOX12, and rhSOX15 is observed, and less than 5% cross-reactivity with rhSOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX8, rhSOX13, rhSOX17, rhSOX18, rhSOX21, and rhSOX30 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6662 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans. Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M Stem Cell Reports, 2015;4(3):419-30. Species: Mouse Sample Type: Whole Cells Application: IHC Not Specified Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up. Authors: Zhang X, Westerhoff M, Hart J Mod Pathol, 2015;28(5):654-61. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column. Authors: Chatterjee S, Sivakamasundari V, Yap S, Kraus P, Kumar V, Xing X, Lim S, Sng J, Prabhakar S, Lufkin T BMC Genomics, 2014;15(0):1072. Species: Mouse Sample Type: Chromatin Application: ChIP Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Authors: Ramasamy S, Kusumbe A, Wang L, Adams R Nature, 2014;507(7492):376-80. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The vanishing testis: a histomorphologic and clinical assessment. Authors: Antic T, Hyjek EM, Taxy JB Am. J. Clin. Pathol., 2011;136(6):872-80. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Identification of SOX3 as an XX male sex reversal gene in mice and humans. Authors: Sutton E, Hughes J, White S J. Clin. Invest., 2011;121(1):328-41. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold. Authors: Cavallo C, Desando G, Facchini A, Grigolo B J Biomed Mater Res A, 2010;93(1):86-95. Species: Human Sample Type: Whole Cells Application: ICC Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines. Authors: Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B Oncogene, 2009;28(35):3121-31. Species: Human Sample Type: Cell Lysates Application: WB A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells. Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM Stem Cells, 2008;0(0):. Species: Human Sample Type: Whole Cells Application: ICC FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD). Authors: Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, Wolffenbuttel KP, Drop SL, Veitia RA, Fellous M, Jaubert F, Looijenga LH J. Pathol., 2008;215(1):31-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human SOX9 Met1-Lys151 Accession # P48436
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Transcription Factors Chondrogenesis Markers Epithelial Stem Cell Transcription Factors Hepatic and Pancreatic Progenitor Cell Transcription Factors High Mobility Group (HMG) Transcription Factors ICC / IHC Images: SOX9 Intestinal Stem Cell Markers Mesenchymal Stem Cell Transcription Factors Neural Stem Cell Markers Neural Stem Cell Transcription Factors Transcription Factors in the Akt Pathway

参考图片

SOX9 in HEK293 Human Cell Line. SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX9 in BG01V Human Embryonic Stem Cells. SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.