Human/Mouse IGF-I R Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse IGF-I R Affinity Purified Polyclonal Ab (25 UG)

货号： AF-305-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-305-SP
别名	CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2
全称	Insulin-like Growth Factor I Receptor
反应种属	Human/Mouse
应用	Western Blot,Immunohistochemistry,Immunocytochemistry,Neutralization
目标/特异性	Detects human and mouse IGF-I R in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 1-15 µg/mL Neutralization: Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 6 ng/mL Recombinant Human IGF‑I.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3480 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies Authors: J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo Cancer Res, 2017;0(0):. Species: Rat Sample Type: Whole Cells Application: Functional Assay Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages Authors: L R Batista-Si Sci Rep, 2016;6(0):27632. Species: Mouse Sample Type: Whole Cells Application: Luciferase Assay Transplantation of Human Neural Progenitor Cells Expressing IGF-1 Enhances Retinal Ganglion Cell Survival. Authors: Ma J, Guo C, Guo C, Sun Y, Liao T, Beattie U, Lopez F, Chen D, Lashkari K PLoS ONE, 2015;10(4):e0125695. Species: Mouse Sample Type: Whole Cells Application: Neut LMP1 promotes expression of insulin-like growth factor 1 (IGF1) to selectively activate IGF1 receptor and drive cell proliferation. Authors: Tworkoski K, Raab-Traub N J Virol, 2015;89(5):2590-602. Species: Human Sample Type: Cell Lysates Application: Neut IGF-IR mediated mammary tumorigenesis is enhanced during pubertal development. Authors: Jones R, Watson K, Campbell C, Moorehead R PLoS ONE, 2014;9(9):e108781. FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival. Authors: Mathur, Anjili, Ware, Christop, Davis, Lenora, Gazdar, Adi, Pan, Bo-Sheng, Lutterbach, Bart PLoS ONE, 2014;9(6):e98515. Species: Human Sample Type: Whole Cells Application: Neut Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD Cancer Res., 2009;69(7):2996-3003. Species: Human Sample Type: Cell Lysates Application: WB Differential expression of receptor tyrosine kinases (RTKs) and IGF-I pathway activation in human uterine leiomyomas. Authors: Yu L, Saile K, Swartz CD, He H, Zheng X, Kissling GE, Di X, Lucas S, Robboy SJ, Dixon D Mol. Med., 2008;14(5):264-75. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	recombinant human IGF-I R extracellular domain Accession # P08069
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.
运输条件	Blue Ice
存放说明	4℃
参考文献	Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

参考图片

IGF‑I R in MCF‑7 and HDLM-2 Human Cell Lines. IGF‑I R was detected in immersion fixed MCF‑7 human breast cancer cell line (left panel) and HDLM‑2 human Hodgkins lymphoma cell line (right panel) using Goat Anti-Human/Mouse IGF‑I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocols for Fluorescent ICC Staining of Cells on CoverslipsandFluorescent ICC Staining of Non-adherent Cells.

IGF‑I R in Human Placenta. IGF‑I R was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 15 µg/mL Goat Anti-Human IGF‑I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑305‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IGF-I R in Mouse Embryo. IGF-I R was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 10 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I R Antibody. Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA). The ND₅₀ is typically 0.5-1.5 µg/mL.