| 货号 | AF-303-SP |
| 别名 | CNTF R alpha; CNTFR; CNTFRa | 全称 | Ciliary Neurotrophic Factor Receptor alpha |
| 反应种属 | Human |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects human CNTF R alpha in direct ELISAs and Western blots. In Western blots, greater than 50% cross-reactivity with recombinant rat CNTF sR alpha is observed and less than 5% cross-reactivity with recombinant human (rh) IL-2 sR gamma and rhIL-6 sR is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CNTF R alpha -enhanced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 1 µg/mL Recombinant Human CNTF R alpha and 20 ng/mL Recombinant Human CNTF. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1271 (Human); 313173 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha Gln23-Pro346 (predicted) Accession # P26992 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutralization by Human CNTF R alpha Antibody. Recombinant Human CNTF R alpha (Catalog # 303‑CR) enhances Recombinant Human CNTF (Catalog # 257-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recombinant Human CNTF R alpha (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑303‑NA). The ND50 is typically 10‑20 µg/mL. |
