Human EphA2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human EphA2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3035-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3035-SP
别名	ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant mouse (rm) EphA2 is observed and less than 1% cross-reactivity with recombinant human (rh) EphA1, rmEphA3, rmEphA4, rhEphA5, rmEphA6, rmEphA7, and rmEphA8 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1969 (Human); 13836 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis. Authors: Gordon K, Kochkodan J, Blatt H, Lin S, Kaplan N, Johnston A, Swindell W, Hoover P, Schlosser B, Elder J, Gudjonsson J, Getsios S J Invest Dermatol, 2013;133(3):712-22. Species: Human Sample Type: tissue Application: IHC Frozen A novel extracellular Hsp90 mediated co-receptor function for LRP1 regulates EphA2 dependent glioblastoma cell invasion. Authors: Gopal U, Bohonowych JE, Lema-Tome C PLoS ONE, 2011;6(3):e17649. Species: Human Sample Type: Whole Cells Application: Flow Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Authors: Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E Mol Biol Cell, 0;27(17):2757-70. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human EphA2 Gln25-Asn534 Accession # P29317
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5 (1-4). A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains (5). The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM) (5). The ECD of human EphA2 shares 90-94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding (6, 7) and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways (8, 9). Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin (10), and can be induced by p53 family transcription factors (11). EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells (12, 13). In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels (14). EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration (13). The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors (15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Poliakov, A. et al. (2004) Dev. Cell 7:465. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1. Bohme, B et al. (1993) Oncogene 8:2857. Pandey, A. et al. (1995) Science 268:567. Bartley, T.D. et al. (1994) Nature 368:558. Pandey, A. et al. (1994) J. Biol. Chem. 269:30154. Miao, H. et al. (2001) Nat. Cell Biol. 3:527. Orsulic, S. and R. Kemler (2000) J. Cell Sci. 113:1793. Dohn, M. et al. (2001) Oncogene 20:6503. Zelinski, D.P. et al. (2001) Cancer Res. 61:2301. Brantley, D.M. et al. (2002) Oncogene 21:7011. Ogawa, K. et al. (2000) Oncogene 19:6043. Sulman, E.P. et al. (1997) Genomics 40:371.

参考图片

Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EphA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3035, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

EphA2 in Human Ovarian Cancer Tissue. EphA2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human EphA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3035) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.