| 货号 | AF3025-SP |
| 别名 | AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta type I receptor; TGFBR1; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I | 全称 | Transforming Growth Factor beta Receptor I |
| 反应种属 | Human |
| 应用 | Western Blot(0.1-0.3 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TGF-beta RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TGF‑ beta RI is observed and less than 5% cross-reactivity with recombinant human (rh) TGF-beta RII and rhTGF-beta RIII is observed. |
| 使用方法 | Western Blot: 0.1-0.3 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7046 (Human); 21812 (Mouse); 29591 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5 Leu34-Glu125 Accession # P36897 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TGF-beta RI, also known as activin-like kinase 5 (ALK-5), is a 55 kDa type I transmembrane serine/threonine receptor kinase. It is ubiquitously expressed and forms a functional heterotetrameric signaling TGF-beta receptor complex with TGF-beta RII. The amino acid sequence of human TGF-beta RI extracellular domain is 91%, 90%, 91%, and 93% identical to that of porcine, mouse, bovine, and rat TGF-beta RI, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human TGF‑ beta RI/ALK‑5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF‑ beta RI/ALK‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF‑ beta RI/ALK‑5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
TGF-beta RI/ALK‑5 in Human PBMCs. TGF-beta RI/ALK‑5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF‑ beta RI/ALK‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
