| 货号 | AF3017-SP |
| 别名 | B14.5b; CI-B14.5b; complex I subunit B14.5b; Complex I-B14.5b; EDM5; HLC-1NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2 (14.5kD, B14.5b); HOA; Human lung cancer oncogene 1 protein; NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa; NADH dehydrogenase [ubiquinone] 1 subunit C2; NADHDH2; NADH-ubiquinone oxidoreductase subunit B14.5b; NDUFC2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Matrilin-3 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Matrilin-3 is observed and less than 1% cross-reactivity with recombinant human (rh) Matrilin-2 and rhMatrilin-4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Matrilin-3 (Catalog # 3017-MN) to immobilized Recombinant Human COMP/Thrombospondin-5 (Catalog # 3134-CP) coated at 2 µg/mL (100 µL/well). At 0.5 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4718 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Matrilin-3 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Matrilin-3 is a 50‑60 kDa extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is primarily expressed in cartilage and functions as a bridging component between proteins of the collagenous matrix (1‑3). The human Matrilin-3 cDNA encodes a 486 amino acid (aa) precursor with a 28 aa signal sequence, an N-terminal VWA domain, four tandem EGF-like repeats, and a C-terminal coiled‑coil domain (4). The Matrilins differ in the number of VWA domains (one or two) and EGF-like repeats (one, three, four, or ten) they contain. Human Matrilin-3 shares 82% aa sequence identity with mouse Matrilin-3. Within the first VWA domain, human Matrilin-3 shares approximately 55% aa sequence identity with human Matrilin-1, -2, and -4. The coiled‑coil domain of Matrilin-3 mediates disulfide-linked homo-oligomerization, with tetramer formation being the most dominant (5‑7). It can also assemble into hetero-oligomers with Matrilin-1 (5‑7). Matrilin-3 is more plentiful than Matrilin-1 in the proliferative zone of the growth plate, whereas the reverse is true in the maturation zone (5). Matrilin-3 interacts directly with Collagen IX and COMP (8, 9). In the absence of Collagen IX, the expression of Matrilin-3 is unchanged, although it is retained inside chondrocytes and is not incorporated into the matrix (9). Matrilin-3 also associates with smaller cartilage fibrils independent of Collagen IX (9). Matrilin-3 knockout mice do not display any obvious abnormalities, suggesting that other molecules may compensate for the lack of Matrilin-3 (10). In contrast, intracellular retention of Matrilin-3 with particular point mutations in the VWA domain results in multiple epiphyseal dysplasia (11‑13). A point mutation in the first EGF-like repeat which has been linked to hand osteoarthritis does not prevent Matrilin-3 secretion (13). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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