Human PI 3-kinase p110 gamma Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human PI 3-kinase p110 gamma Affinity Purified Polyclonal Ab (25 UG)

货号： AF2999-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2999-SP
别名	1-phosphatidylinositol 3-kinase; EC 2.7.1; EC 2.7.1.153; p110-gamma; p120-PI3K; phosphatidylinositol 3 kinase gamma, p110 gamma; phosphatidylinositol 3-kinase catalytic 110-kD gamma; phosphatidylinositol 3-kinase, catalytic, gamma polypeptide; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; phosphoinositide-3-kinase gamma catalytic subunit; phosphoinositide-3-kinase, catalytic, gamma polypeptide; PI3CG; PI3K; PI3K-gamma ; PI3Kgamma; PI3K-gamma; PI3-kinase subunit gamma; PIK3; PIK3CG; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma
全称	Protein Tyrosine Phosphatase 1G
反应种属	Human
应用	Western Blot(2 µg/mL)
目标/特异性	Detects human PI 3‑Kinase p110 gamma in direct ELISAs and Western blots.
使用方法	Western Blot: 2 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5294 (Human); 30955 (Mouse); 298947 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human PI 3‑Kinase p110 gamma Asn298-Leu467 Accession # P48736
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	p110-gamma (protein kinase 110 kDa gamma; also PI3 kinase subunit gamma, Ser/Thr protein kinase PIK3CG and Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma) is a 110-120 kDa Class I member of the PI3/PI4-kinase family of molecules. Schematically, PI3K (PI3 kinase) is typically thought of as a heterodimeric complex composed of one p85, p101 or p84 regulatory subunit coupled to one p110 catalytic subunit. In response to receptor tyrosine kinase ligation, PI3K is recruited to a transmembrane kinase receptor where it is activated. Active PI3K subsequently catalyzes the phosphorylation of phosphatidylinositol, generating a molecule (PIP3) that mediates downstream signaling. p110 gamma is one of four p110 genes that generates PIP3. Unlike p110 alpha, beta and δ, however, it neither interacts with activated Tyr kinases nor with p85. Instead, it associates with either p101 or p84, and interacts with the GPCR G beta gamma subunits to mediate downstream signaling. Human p110 gamma is 1102 amino acids in length. It contains one ABD (adaptor-binding domain) (aa 34-141), an RBD (ras-binding domain) (217-309), a C2 PI3K‑type domain (aa 357-521), a substrate presentating PIK domain (aa 549-725) and a C-terminal catalytic region (aa 830-1046). There is one utilized phosphorylation site at Ser1101. Over aa 298-467, human p110 gamma shares 94% aa sequence identity with mouse p110 gamma.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family Diabetic Peripheral Neuropathy GDNF Family Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family Notch Pathway PDGF Family Signaling Molecules and Transcriptional Regulators of Tfh Cell Differentiation TNF Superfamily Regulators VEGF Family

参考图片

Detection of Human PI 3‑Kinase p110 gamma by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human PI 3‑Kinase p110 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PI 3‑Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.