| 货号 | AF2987-SP |
| 别名 | EC 2.7.11; EC 2.7.11.1; KLS; p70 ribosomal S6 kinase beta; p70 S6 kinase beta; p70 S6KB; p70 S6K-beta; p70(S6K)-beta; p70-beta; P70-beta-1; P70-beta-2; p70-S6K 2; P70S6K2; p70S6Kb; ribosomal protein S6 kinase beta-2; ribosomal protein S6 kinase, 70kD, polypeptide 2,70 kDa ribosomal protein S6 kinase 2; ribosomal protein S6 kinase, 70kDa, polypeptide 2; RPS6KB2; S6K2; S6K-beta; S6K-beta2; S6K-beta-2; serine/threonine-protein kinase 14 beta; Serine/threonine-protein kinase 14B; SRK; STK14B; STK14BS6 kinase-related kinase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human p70 S6 Kinase beta in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human p70 S6 Kinase is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6199 (Human); 58988 (Mouse); 361696 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human p70 S6 Kinase beta Ala2-Ser410 Accession # Q9UBS0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | P70S6K beta (p70 ribosomal S6 kinase beta-2; also RPS6KB2, S6K beta, S6K beta 2, S6K2 and STK14B) is a monomeric, 54 kDa member of the AGC family of Ser/Thr kinases. It is widely expressed, and found predominately in the nucleus. Although it was initially reported to phosphorylate the ribosomal S6 protein, p70S6K beta /S6K beta 2 is now known to also phosphorylate RafB (cell survival), hnRNP (cell proliferation), p300/CBP (transcription) and CDC42 (cytoskeleton stability). Human p70S6K beta /S6K beta 2 is 482 amino acids in length. It contains a protein kinase domain (aa 67-328), a C-terminal regulatory region (aa 329-399) that contains an NLS (aa 384-389), and a Pro-rich segment (aa 411-482) that likely interacts with SH3 domain-containing proteins. Phosphorylation/dephosphorylation events and sumoylation regulate p70S6K beta activity. In addition to the 54 kDa form, there is a 56-60 kDa isoform that possesses a 13 aa addition at the N-terminus, plus an isoform that shows a deletion of aa 267-270. Over aa 1-410, human p70S6K beta 2 shares 95% and 75% aa identity with mouse p70S6K beta 2and human p70S6K beta 1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human p70 S6 Kinase beta by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p70 S6 Kinase beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
p70 S6 Kinase beta in MCF‑7 Human Cell Line. p70 S6 Kinase beta was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
