Human NRG1-alpha/HRG1-alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human NRG1-alpha/HRG1-alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF-296-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-296-SP
别名	ARIA; GGF2; HGL; HRG; HRG1; MST131; MSTP131; neuregulin 1; Neuregulin1 alpha; Neuregulin-1 alpha; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF
反应种属	Human
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects human NRG1-alpha /HRG1-alpha in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize NRG1‑ alpha /HRG1‑ alpha -induced proliferation in the MCF‑7 human breast cancer cell line. The Neutralization Dose (ND₅₀) is typically 7-30 µg/mL in the presence of 125 ng/mL Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3084 (Human); 211323 (Mouse); 112400 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Type I neuregulin1? is a novel local mediator to suppress hepatic gluconeogenesis in mice Authors: T Arai, Y Ono, Y Arimura, K Sayama, T Suzuki, S Shinjo, M Kanai, SI Abe, K Semba, N Goda Sci Rep, 2017;7(0):42959. Species: Mouse Sample Type: Tissue Homogenates Application: WB Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke. Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO Neuropharmacology, 2010;59(7):640-9. Species: Human Sample Type: Recombinant Protein Application: WB HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors. Authors: Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II Clin. Cancer Res., 2009;15(15):4829-37. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human NRG1-alpha /HRG1-alpha Ser177-Lys241 Accession # Q7RTV8
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1encodes more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. The alpha - or beta -splice variants differ in their C-terminal region. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF). NRG1s have an immunoglobulin (Ig)-like domain N‑terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1 (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and to promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.
运输条件	Blue Ice
存放说明	4℃
参考文献	Lemke, G. (2006) Science STKE 325:pe11. Esper, R.M. et al. (2006) Brain Res. Brain Res. Rev. 51:161. Tosato, S. et al. (2005) Schizophr. Bull. 31:613.

参考图片

Cell Proliferation Induced by NRG1‑ alpha /HRG1‑ alpha and Neutralization by Human NRG1‑ alpha /HRG1‑ alpha Antibody. Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain (Catalog # 296-HR) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain (125 ng/mL) is neutralized (green line) by increasing concentrations of Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-296-NA). The ND₅₀ is typically 7-30 µg/mL.

NRG1-alpha /HRG1-alpha in Human Breast. NRG1-alpha /HRG1-alpha was detected in paraffin-embedded sections of human breast using Goat Anti-Human NRG1-alpha /HRG1-alpha EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑296‑NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.