| 货号 | AF2964-SP |
| 别名 | FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein | 全称 | Fatty Acid-Binding Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.25 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FABP1/L‑FABP in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant rat FABP1 and less than 5% cross-reactivity with recombinant human FABP2, 3, 4, 5, 7, and recombinant mouse FABP9 is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2168 (Human); 14080 (Mouse); 24360 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FABP1/L‑FABP Met1-Ile127 Accession # P07148 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FABP1, also known as liver FABP (L-FABP, Z-protein. and squalene and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney, and lung. FABP1 binds free fatty acids, their co-enzyme A derivatives, and may be involved in intracellular lipid transport. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human FABP1/L‑FABP by Western Blot. Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human FABP1/L‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
FABP1 in Human Liver. FABP1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human FABP1/L‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
