Human HGF Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human HGF Affinity Purified Polyclonal Ab (25 UG)

货号： AF-294-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-294-SP
别名	deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
全称	Hepatocyte Growth Factor
反应种属	Human
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects human HGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse HGF is observed. In Western blots, no cross-reactivity with recombinant canine HGF is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize HGF-induced IL-11 secretion in the Saos‑2 human osteosarcoma cell line. Hjertner, O. et al. (1999) Blood 94:3883.The Neutralization Dose (ND₅₀) is typically 0.01-0.06 μg/mL in the presence of 3 ng/mL Recombinant Human HGF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3082 (Human); 15234 (Mouse); 403441 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Matriptase regulates c-Met mediated proliferation and invasion in inflammatory breast cancer Authors: GL Zoratti, LM Tanabe, TE Hyland, MJ Duhaime, É Colombo, R Leduc, E Marsault, MD Johnson, CY Lin, J Boerner, JE Lang, K List Oncotarget, 2016;7(36):58162-58173. Species: Human Sample Type: Cell Lysates Application: WB Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K Nat Commun, 2015;6(0):6776. Species: Human Sample Type: Cell Culture Supernates Application: WB Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET. Authors: Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M J Biol Chem, 2014;289(16):11068-82. Species: Human Sample Type: Whole Cells Application: Neut Functional mononucleotide repeat polymorphism in the promoter region of HGF is associated with risk and malignant aggressiveness of bladder cancer. Authors: Chiba S, Tsuchiya N, Horikawa Y, Narita S, Inoue T, Akihama S, Saito M, Numakura K, Tsuruta H, Huang M, Satoh S, Habuchi T Int J Oncol, 2014;44(3):678-84. Species: Human Sample Type: Whole Tissue Application: IHC - paraffin Spontaneous genomic alterations in a chimeric model of colorectal cancer enable metastasis and guide effective combinatorial therapy. Authors: Zhou Y, Rideout W, Bressel A, Yalavarthi S, Zi T, Potz D, Farlow S, Brodeur J, Monti A, Reddipalli S, Xiao Q, Bottega S, Feng B, Chiu M, Bosenberg M, Heyer J PLoS ONE, 2014;9(8):e105886. Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma. Authors: Imura, Yoshinor, Yasui, Hirohiko, Outani, Hidetats, Wakamatsu, Toru, Hamada, Kenichir, Nakai, Takaaki, Yamada, Shutaro, Myoui, Akira, Araki, Nobuhito, Ueda, Takafumi, Itoh, Kazuyuki, Yoshikawa, Hideki, Naka, Norifumi Mol Cancer, 2014;13(0):185. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Associations between hepatocyte growth factor, c-Met, and basic fibroblast growth factor and survival in endometrial cancer patients. Authors: Felix AS, Edwards RP, Stone RA Br. J. Cancer, 2012;106(12):2004-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity. Authors: Ramani VC, Yang Y, Ren Y J. Biol. Chem., 2011;286(8):6490-9. Species: Human Sample Type: Cell Culture Supernates Application: IP c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase. Authors: Szabo R, Rasmussen AL, Moyer AB Oncogene, 2011;30(17):2003-16. Species: Human Sample Type: Cell Culture Supernates Application: WB Wnt activity defines colon cancer stem cells and is regulated by the microenvironment. Authors: Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP Nat. Cell Biol., 2010;12(5):468-76. Species: Human Sample Type: Whole Cells Application: ICC Alternative proteolytic processing of hepatocyte growth factor during wound repair. Authors: Buchstein N, Hoffmann D, Smola H, Lang S, Paulsson M, Niemann C, Krieg T, Eming SA Am. J. Pathol., 2009;174(6):2116-28. Species: Human Sample Type: Tissue Secretion Application: WB Endogenous hepatocyte growth factor is a niche signal for subventricular zone neural stem cell amplification and self-renewal. Authors: Nicoleau C, Benzakour O, Agasse F, Thiriet N, Petit J, Prestoz L, Roger M, Jaber M, Coronas V Stem Cells, 2008;0(0):. Species: Canine Sample Type: Whole Cells Application: Neut IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism. Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K Stem Cells, 2008;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Authors: Skibinski G, Elborn JS, Ennis M Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76. Species: Human Sample Type: Whole Cells Application: Neut Hepatocyte growth factor acts as a motogen and guidance signal for gonadotropin hormone-releasing hormone-1 neuronal migration. Authors: Giacobini P, Messina A, Wray S, Giampietro C, Crepaldi T, Carmeliet P, Fasolo A J. Neurosci., 2007;27(2):431-45. Species: Mouse Sample Type: Tissue Homogenates Application: WB Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome. Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H J Trauma, 2004;56(2):259-64. Species: Human Sample Type: Cell Culture Supernates Application: WB Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1. Authors: Kawai A, Naito N, Ouchida M, Beppu Y Cancer Lett., 2004;204(1):105-13. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF Accession # P14210
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adhesion Molecules in Angiogenesis Cytokines and Receptors in Angiogenesis EMT Induction Gametes and Fertilization Hepatic Endoderm Integrins and Associated Molecules Pancreatic Endoderm Preeclampsia Semaphorins, Plexin Receptors, and Related Molecules

参考图片

Detection of Recombinant Human HGF by Western Blot. Western blot shows 25 ng of Recombinant Human HGF (Catalog #
294‑HG), Recombinant Mouse HGF (Catalog # 2207-HG) and Recombinant Canine HGF (Catalog # 3386-HG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for HGF at approximately 30 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

HGF in Human Liver.

HGF was detected in formalin fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑294‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody.

Recombinant Human HGF induces IL-11 secretion in the Saos‑2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibod (Catalog #
AF-294-NA). The ND₅₀ is typically 0.01‑0.06 μg/mL.