Human CMG-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CMG-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2940-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2940-SP
别名	anthrax toxin receptor 2; ANTXR2; Capillary morphogenesis gene 2 protein; capillary morphogenesis protein 2; cI-35; CMG2MGC111533; CMG-2MGC45856; FLJ31074; ISH; JHF; JHS
全称	Capillary Morphogenesis Protein 2
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human CMG‑2/ANTXR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse CMG-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	118429 (Human); 71914 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential dependence on N-glycosylation of anthrax toxin receptors CMG2 and TEM8. Authors: Friebe S, Deuquet J, van der Goot F PLoS ONE, 2015;10(3):e0119864. Species: Human Sample Type: Whole Cells Application: IP Therapeutic potential of capillary morphogenesis gene 2 extracellular vWA domain in tumourrelated angiogenesis. Authors: Ye L, Sun P, Sanders A, Martin T, Lane J, Mason M, Jiang W Int J Oncol, 2014;45(4):1565-73. Species: Human Sample Type: Total protein Application: WB Bacillus anthracis lethal toxin reduces human alveolar epithelial barrier function. Authors: Langer, Marybeth, Duggan, Elizabet, Booth, John Lel, Patel, Vineet I, Zander, Ryan A, Silasi-Mansat, Robert, Ramani, Vijay, Veres, Tibor Zo, Prenzler, Frauke, Sewald, Katherin, Williams, Daniel M, Coggeshall, Kenneth, Awasthi, Shanjana, Lupu, Florea, Burian, Dennis, Ballard, Jimmy Da, Braun, Armin, Metcalf, Jordan P Infect Immun, 2012;80(12):4374-87. Species: Human Sample Type: Cell Lysates Application: WB Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human CMG‑2/ANTXR2 isoform 1 Gln34-Asn317 Accession # P58335
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1‑3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoforms 4 shows a 12 aa insertion in the cytoplasmic region; isoform 2 shows a 103 aa deletion in the ECD; and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1‑5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence homology with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.
运输条件	Blue Ice
存放说明	4℃
参考文献	Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367. Santelli, E. et al. (2004) Nature 430:905. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145. Abrami, L. et al. (2006) J. Cell Biol. 172:309. Wei, W. et al. (2006) Cell 124:1141. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.

参考图片

Detection of CMG‑2/ANTXR2 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Human CMG‑2/ANTXR2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2940, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).