Human VEGF 165 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human VEGF 165 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-293-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-293-SP
别名	MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
全称	Vascular Endothelial Growth Factor
反应种属	Human
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects human VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant canine VEGF is observed, and less then 20% cross-reactivity with recombinant mouse VEGF₁₆₅ and recombinant rat VEGF₁₆₄ is observed, and less than 5% cross-reactivity with recombinant zebrafish VEGF is observed.
使用方法	Western Blot: 5-10 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize VEGF₁₆₅-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The Neutralization Dose (ND₅₀) is typically 0.02‑0.12 µg/mL in the presence of 10 ng/mL Recombinant Human VEGF₁₆₅.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells Authors: S Yang, F Qi, C Tang, H Wang, H Qin, X Li, J Li, W Wang, C Zhao, H Gao Oncol Lett, 2017;13(2):898-904. Species: Human Sample Type: Whole Cells Application: ICC Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard PLoS ONE, 2017;12(9):e0184039. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Capture Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung Nat Commun, 2017;8(0):14399. Species: Human Sample Type: Whole Cells Application: Neut PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li Oncotarget, 2016;7(47):77902-77915. Species: Human Sample Type: Whole Cells Application: Neut Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling. Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J Mol Ther, 2014;22(7):1243-53. Species: Mouse Sample Type: In Vivo Application: In vivo Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype. Authors: Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger K, Zambruno G, Failla C FASEB J, 2014;28(2):692-704. Species: Human Sample Type: Whole Cells Application: Neut Detection of VEGF-A(xxx)b isoforms in human tissues. Authors: Bates, David O, Mavrou, Athina, Qiu, Yan, Carter, James G, Hamdollah-Zadeh, Maryam, Barratt, Shaney, Gammons, Melissa, Millar, Ann B, Salmon, Andrew H, Oltean, Sebastia, Harper, Steven J PLoS ONE, 2013;8(7):e68399. Species: Human Sample Type: Cell Lysates Application: IP Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness. Authors: Ruffini F, Tentori L, Dorio A, Arcelli D, DAmati G, DAtri S, Graziani G, Lacal P Oncol Rep, 2013;30(6):2887-96. Simple patterned nanofiber scaffolds and its enhanced performance in immunoassay. Authors: Wang, Jing, Kang, Qin-shu, Lv, Xiao-gua, Song, Jia, Zhan, Na, Dong, Wei-guo, Huang, Wei-hua PLoS ONE, 2013;8(12):e82888. Species: Human Sample Type: Recombinant Protein Application: micro-immunoassay development Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development Chronic exposure of colorectal cancer cells to bevacizumab promotes compensatory pathways that mediate tumour cell migration. Authors: Fan F, Samuel S, Gaur P Br. J. Cancer, 2011;104(8):1270-7. Species: Human Sample Type: Cell Lysates Application: WB Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model. Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST Angiogenesis, 2010;13(4):337-47. Species: Human Sample Type: Whole Cells Application: Neut VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo. Authors: Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ Br. J. Cancer, 2009;101(7):1183-93. Species: Human Sample Type: Cell Lysates Application: WB The hypoxia-inducible factor 1/NOR-1 axis regulates the survival response of endothelial cells to hypoxia. Authors: Martorell L, Gentile M, Rius J, Rodriguez C, Crespo J, Badimon L, Martinez-Gonzalez J Mol. Cell. Biol., 2009;29(21):5828-42. Species: Human Sample Type: Whole Cells Application: Neut Effect of chemotherapeutic stress on induction of vascular endothelial growth factor family members and receptors in human colorectal cancer cells. Authors: Fan F, Gray MJ, Dallas NA, Yang AD, Van Buren G, Camp ER, Ellis LM Mol. Cancer Ther., 2008;7(9):3064-70. Species: Human Sample Type: Cell Lysates Application: WB Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1. Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S J. Biol. Chem., 2008;283(49):34250-9. Species: Human Sample Type: Recombinant Protein Application: ELISA Development Comparison of bevacizumab, ranibizumab, and pegaptanib in vitro: efficiency and possible additional pathways. Authors: Klettner A, Roider J Invest. Ophthalmol. Vis. Sci., 2008;49(10):4523-7. Species: Porcine Sample Type: Cell Lysates Application: WB Mammary alveolar development during lactation is inhibited by the endogenous antiangiogenic growth factor isoform, VEGF165b. Authors: Qiu Y, Bevan H, Weeraperuma S, Wratting D, Murphy D, Neal CR, Bates DO, Harper SJ FASEB J., 2007;22(4):1104-12. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment. Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA Blood, 2007;109(1):122-9. Species: Human Sample Type: Whole Tissue Application: ICC Bactericidal/permeability-increasing protein's signaling pathways and its retinal trophic and anti-angiogenic effects. Authors: Yamagata M, Rook SL, Sassa Y, Ma RC, Geraldes P, Goddard L, Clermont A, Gao B, Salti H, Gundel R, White M, Feener EP, Aiello LP, King GL FASEB J., 2006;20(12):2058-67. Species: Bovine Sample Type: Cell Culture Supernates Application: ELISA Development Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells. Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB Glia, 2006;53(8):845-57. Species: Human Sample Type: Whole Cells Application: Neut Inhibition of cyclooxygenase-2 disrupts tumor vascular mural cell recruitment and survival signaling. Authors: Lee A, Frischer J, Serur A, Huang J, Bae JO, Kornfield ZN, Eljuga L, Shawber CJ, Feirt N, Mansukhani M, Stempak D, Baruchel S, Bender JG, Kandel JJ, Yamashiro DJ Cancer Res., 2006;66(8):4378-84. Species: Human Sample Type: Whole Cells Application: ICC The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term. Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ Clin. Sci., 2006;110(5):575-85. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development Age-associated aortic stenosis in apolipoprotein E-deficient mice. Authors: Tanaka K, Sata M, Fukuda D, Suematsu Y, Motomura N, Takamoto S, Hirata Y, Nagai R J. Am. Coll. Cardiol., 2005;46(1):134-41. Species: Mouse Sample Type: Whole Tissue Application: IHC The proline-rich homeodomain (PRH/HEX) protein is down-regulated in liver during infection with lymphocytic choriomeningitis virus. Authors: Djavani M, Topisirovic I, Zapata JC, Sadowska M, Yang Y, Rodas J, Lukashevich IS, Bogue CW, Pauza CD, Borden KL, Salvato MS J. Virol., 2005;79(4):2461-73. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Tissue Application: IHC Paraffin-embedded Human peripheral blood eosinophils induce angiogenesis. Authors: Puxeddu I, Alian A, Piliponsky AM, Ribatti D, Panet A, Levi-Schaffer F Int. J. Biochem. Cell Biol., 2005;37(3):628-36. Species: Chicken Sample Type: Whole Tissue Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath. Authors: Vijayan V, Shukla N, Johnson JL, Gadsdon P, Angelini GD, Smith FC, Baird R, Jeremy JY J. Vasc. Surg., 2004;40(5):1011-9. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries. Authors: Wright JL, Tai H, Churg A Am. J. Respir. Cell Mol. Biol., 2004;31(5):501-9. Species: Guinea Pig Sample Type: Whole Tissue Application: IHC Paraffin-embedded Estrogen enhances angiogenesis through a pathway involving platelet-activating factor-mediated nuclear factor-kappaB activation. Authors: Seo KH, Lee HS, Jung B, Ko HM, Choi JH, Park SJ, Choi IH, Lee HK, Im SY Cancer Res., 2004;64(18):6482-8. Species: Porcine Sample Type: Whole Cells Application: Neut Alterations in angiogenic growth factors and neuronal nitric oxide synthase expression in chronic cavernosal ischemia. Authors: Wang T, Soker S, Atala A, Siroky MB, Azadzoi KM Int. J. Impot. Res., 2004;16(5):403-11. Species: Rabbit Sample Type: Whole Tissue Application: IHC Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops. Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS Int. J. Cancer, 2003;104(5):603-10. Species: Human Sample Type: Cell Lysates Application: WB Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V Blood, 2003;102(2):638-45. Species: Human Sample Type: Whole Cells Application: Neut VEGF signaling is required for the assembly but not the maintenance of embryonic blood vessels. Authors: Argraves WS, Larue AC, Fleming PA, Drake CJ Dev. Dyn., 2002;225(3):298-304. Species: Avian - Quail Sample Type: In Vivo Application: In vivo Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome. Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM J. Immunol., 2002;169(11):6515-21. Species: Human Sample Type: BALF Application: ELISA Development VEGF in the lung: a role for novel isoforms. Authors: Varet, Julia, Varet J, Douglas SK, Douglas, Samantha, Gilmartin, Laura, Gilmartin L, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B Am J Physiol Lung Cell Mol Physiol, 0;298(6):L768-74. Species: Human Sample Type: BALF Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF₁₆₅ Ala27-Arg191 Accession # NP_001165097.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF is a soluble protein secreted by a wide variety of cell types. Itbinds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2(Flk-1). VEGF stimulates vascular endothelial cell proliferation and isa potent inducer of angiogenesis. Several VEGF isoforms occur resultingfrom alternative mRNA splicing.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Adult Neurogenesis Airway Inflammation and Remodeling ALS Related Molecules Cancer Biomarkers Developmental Factors Implicated in Neurodegeneration HIF Transcription Factors Inflammatory Mediators Macrophage Activation Markers Myeloid-derived Suppressor Cells (MDSC) Platelets Preeclampsia VEGF Family VEGF Family Ligands

参考图片

VEGF in HUVEC Cells. VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF₁₆₅ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 5-10 µg/mL of Goat Anti-Human VEGF₁₆₅ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VEGF at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by VEGF₁₆₅ and Neutralization by Human VEGF Antibody. Recombinant Human VEGF₁₆₅(Catalog #293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF₁₆₅ (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF₁₆₅ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑293‑NA). The ND₅₀ is typically 0.02-0.12 µg/mL.