| 货号 | AF2939-SP |
| 别名 | CA6; Carbonate dehydratase VI; carbonic anhydrase 6; carbonic anhydrase VIGUSTIN; CA-VI; EC 4.2.1.1; Gustin; MGC21256; Salivary carbonic anhydrase; Secreted carbonic anhydrase |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase (CA) 5A and rhCA5B is observed and less than 1% cross-reactivity with rhCA1, rhCA2, rhCA3, rhCA4, rhCA7, rhCA8, rhCA9, rhCA10, rhCA12, rhCA13, and rhCA14 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 765 (Human); 298657 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI Gln18-Asn308 Accession # EAW71606 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Carbonic Anhydrase VI by Western Blot. Western blot shows human saliva, human milk, and lysates of HSC-3 human oral squamous cell carcinoma cell line and LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Carbonic Anhydrase VI by Simple WesternTM. Simple Western lane view shows lysates of human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
