Human IGF-II Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IGF-II Affinity Purified Polyclonal Ab (25 UG)

货号： AF-292-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-292-SP
别名	C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PP9974; somatomedin-A
全称	Insulin-like Growth Factor II
反应种属	Human
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects human IGF-II in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IGF‑II is observed and less than 1% cross-reactivity with recombinant human IGF-I is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IGF‑II-induced proliferation in the MCF‑7 human breast cancer cell line [Karey, K.P. et al. (1988) Cancer Research 48:4083]. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 14 ng/mL Recombinant Human IGF‑II.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3481 (Human); 16002 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung Nat Commun, 2017;8(0):14399. Species: Human Sample Type: Whole Cells Application: Neut A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line. Authors: Benabbou N, Mirshahi P, Bordu C, Faussat A, Tang R, Therwath A, Soria J, Marie J, Mirshahi M Int J Oncol, 2014;45(4):1372-80. 'Big'-insulin-like growth factor-II signaling is an autocrine survival pathway in gastrointestinal stromal tumors. Authors: Rikhof B, van der Graaf WT, Suurmeijer AJ, van Doorn J, Meersma GJ, Groenen PJ, Schuuring EM, Meijer C, de Jong S Am. J. Pathol., 2012;181(1):303-12. Species: Human Sample Type: Tissue Homogenates Application: WB Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development Insulin-like growth factor-II is increased in systemic sclerosis-associated pulmonary fibrosis and contributes to the fibrotic process via Jun N-terminal kinase- and phosphatidylinositol-3 kinase-dependent pathways. Authors: Hsu E, Feghali-Bostwick CA Am. J. Pathol., 2008;172(6):1580-90. Species: Human Sample Type: Whole Cells Application: ICC The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori. Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A Gastroenterology, 2006;130(6):1754-63. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IGF-II Ala25-Glu91 Accession # P01344
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine, and porcine proteins) and exhibit cross-species activity. IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development. Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Diabetes and Obesity Research HIF Transcription Factors IGF Family IGF Ligands Insulin-like Growth Factor (IGF) and Receptors Metabolic Peptide Hormones and Regulators Protein/Peptide Hormones and Regulators Reproduction

参考图片

Cell Proliferation Induced by IGF‑II and Neutralization by Human IGF‑II Antibody.

Recombinant Human IGF‑II (Catalog # 292-G2) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑II (14 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-292-NA). The ND₅₀ is typically 0.2-0.8 µg/mL.

IGF‑II in Human Placenta.

IGF‑II was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGF‑II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑292‑NA) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.